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08 June 2001 @ 09:34 pm
Loooong Week  
This week seems to have lasted forever. I've been pretty brain-dead all week, but I managed to get a fair bit of work done at the beginning of it. I got some Kiapp data that Vera wants for a paper, amongst other things. Later in the week this work lark started to come apart somewhat - I didn't do very much yesterday or today. Partly because the fluorimeters were playing up. Two problems really. Firstly, I'm doing kons for this TIMP complex that Vera wants data for. These produce nice curves that you fit to some horrendously complex equation. But my curves were dipping (impossible - implies the enzyme is creating substrate from product, and OK so all reactions are reversible but this one isn't that reversible) and coming back up again. That one turned out to be the magnetic fleas (stirrer bars) in the cuvettes, dunno precisely how/why but they were screwing things up. And the other problem is that one of the machines has some disconnect between the software and the hardware (no, we don't know why - it's the same software and same type of hardware as the other two, but it's got 'issues'), so you get communication errors after a couple of thousand seconds or so, which isn't much good if you wanna do a run for about ten thousand seconds. So next week I get to repeat those experiments with a different machine and no fleas (and hope diffusion doesn't screw over the results too much).

The other reason that I didn't get much done today was that we stayed up to watch some of the election coverage and I got about 4.5 hours sleep. This does me no good - I look awake, I sound awake but I have the concentration span of a hyperactive 2 year old and the mental abilities of an infant. I had about 3 cups of coffee during the day (which didn't affect my digestive system - so hopefully my over sensitivity to caffeine is getting back to normality - yay!), and I'm still in that sort of 'lights are on but nobody's home' state.

Went out to vote last night and, as J says, it was a little anti-climactic. Particularly as Suffolk Central & North Ipswich is a strong Tory seat, so in many ways our votes counted for nothing. Didn't have as much hassle as sagima did, dunno why though - probably coz we're a little older. It's only the second general election I've been old enough to vote in (I missed the one before by 2 months) so it's still a little exciting to actually get to vote, despite the anti-climax. And despite the fact that I'm getting quite deeply cynical about New Labour having had them in power for the last 4 years. Don't get me started on public transport ;)

Other happenings at work - a computer died. Like, dead. Though one of the IT support staff has fixed it - but I'm not convinced it'll stay fixed. Basically C: was so corrupt that NT wouldn't boot. The boot sector was fine, but as soon as it actually started to boot NT it hung. The tech has reformated the drive and reinstalled - but I'm worried that we still don't know why it got corrupted. Or how for that matter.

We also got going with some cell culture. Nik and I are transfecting some cells with TIMP3. We think it's not going to work this time though :/ The protocol we're following is for a slightly different procedure (transient transfections as opposed to stable transfections), and although we've altered most stuff suitably we think we've got too high a cell number. So we may be starting again with fewer cells on Monday.

Oh and people were as I expect them to be. We had a little example of idiocy the other day - of someone's Palm it was said "I used to wonder who was taken in by the advertising rubbish, now I know it's gullible boys". No, obviously there can be no possible reason to get a Palm other than because you saw an advert. Oh, no. Grr.
Current Mood: tiredtired
Current Music: None - sound from the telly instead
gabby776 on June 9th, 2001 08:14 am (UTC)
I hope that your weekend includes something fun to make up for all the work you've done this past week. I wish I even understood a little of what you do at work. It sounds complex and tedious to me. I really like your photo, btw. :) It's a good picture of you :)
Margaretpling on June 10th, 2001 01:43 am (UTC)
Thanks :)
Ceci n'est pas une viemarble on June 9th, 2001 08:42 am (UTC)
"Windows happens"
-- Based on a similar quote
Margaretpling on June 10th, 2001 03:00 am (UTC)

Yeah. And don't you just hate it when people expect you to make it all better, but still want to use the damn thing ;)
Ceci n'est pas une viemarble on June 10th, 2001 06:06 am (UTC)
Yes. (And I don't think we're alone.)
Sathsath on June 10th, 2001 02:45 am (UTC)
Public Transport?
I actually like public transport quite a lot. I distinctly remember waiting at the train station with Chess for her train home and I was just grinning round insanely saying 'Wow isn't public transport wonderful? I really think it is' and I swear she thought I was delerious. And I do love public transport, it gets you places, which makes my life a whole lot better, and I dont have any complaints about really long delays because I can quite well entertain myself for hours and hours.

*waits to be growled at by those who dont have the same view as her*
Margaretpling on June 10th, 2001 02:52 am (UTC)
Re: Public Transport?
Public transport is good in concept, it's just the current implementation that bugs me. Given I need to use 3 different instances of public transport every time I go to or come home from work delays and missing buses/trains do bug me. If the first bus cocksup then I miss the train etc etc. And the current mainline train service is actually worse now than the local line I used to use 5 years ago (which is pretty crap in itself, now). Given I give the various companies involved something like £3,000 a year between them (and I have a 'cheap' train journey) I expect somewhat better service, and certainly not deterioration over the same period as price rises.
Ceci n'est pas une viemarble on June 10th, 2001 06:02 am (UTC)
Re: Public Transport?
Growling at people who don't share your views is a strange and silly concept. What I really clicked on the 'reply' link for though was to say, "You don't drive yet, do you?" :)
Sathsath on June 10th, 2001 07:47 am (UTC)
Re: Public Transport?
What you really wanted to ask was 'do you have a driving licsence yet' to which I could answer 'no'.
I live in a place where its perfectly legal for me to drive. And if you ask Niri she was lovely and took me out for my first lesson down the airfield when I was 13 (I think it was 13, could have been 14).

But still when I get my licscence Im not going to drive a car unless Im taking at least one person with me. Cos otherwise its a waste. public transport it much better.