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24 July 2001 @ 07:51 pm
Been busy busy at work the last coupla days. The purification stuff is winding down - I'm doing the last few concentrations and active site titrations now. Hopefully I'll not have to do this again for a while *fingers crossed*.

We're still going with the transfection too - but Nik's away, so I'm doing all the work this week. It's not too hard though, just changing medium on the 96 well plates three times this week, and keeping a couple of cell lines going in case it all goes wrong.

And I've done some more work on the project that I'm 'supposed' to be working on ... my PCR still isn't working, but Phil (PhD student with the bench next to me who's done a fair amount of PCR in the past) had a couple of suggestions. I tried one today and it didn't work, and I've done the set up for her other suggestion, so I'll see if that works tomorrow. If it doesn't work, then it's beyond my ability to troubleshoot (ditto Phil's) so I'll have to go talk to the boss. Which is kinda admitting defeat, but defeated I will be, so I might as well admit it ;)

I also read a chapter of a book that I need to incorporate into my thesis somehow ... all about exosites in MMPs, and how they affect kinetics, particularly collagenolysis. I found something to put in where Vera had indicated I needed to, and I think I don't need to mention it anywhere else - which is good, coz at first I thought it might involve major re-writes of the discussion. But I've decided I disagree with too much of what he says about the methodology so I don't particularly want to get drawn on it in the viva. Justifiably so I might add - he goes on and on about how single amino acid mutations are the only way to go. But we're talking about a 400 amino acid protein here - and all the bits are implicated in collagenolysis so where the hell do you start??? You've got to narrow it down to particular regions before you start just changing an occassional residue. But although it's justifiable, I don't want it to be a topic of conversation if it doesn't have to be ;)

Previous book | "The Dark Moon" (Book 1 of the Guardian Cycle) by Julia Gray
Current book | "First Contract: A Novel of Big Business and Alien Invasion" by Greg Costikyan
Current Webscriptions book | "Bolo Strike" by William H. Keith Jr.
Current Mood: cheerfulcheerful
Current Music: GT3 on the PS2
Clearly Canadian :)canuckgirl on July 24th, 2001 12:21 pm (UTC)

A question for you. What's a bedsit (or bedsitter)?? I'm thinking it's some type of apartment (flat), that you pay rent on??
Margaretpling on July 24th, 2001 12:52 pm (UTC)
It's one room, that you pay rent to live in. You share the bathroom with the other bedsits in the house, but otherwise you just have the one room. So the 'kitchen' is in there, and your bed, and whatever other furniture you want to have. My brother lives in one (they're generally cheap) and he has two rings to cook on (so does a lot of chinese or thai stirfry) and just about enough room for bed, wardrobe and desk.
Clearly Canadian :)canuckgirl on July 24th, 2001 01:39 pm (UTC)

Sharing a bathroom?? Ick!

But it does sound pretty cheap.
Andrewsagima on July 25th, 2001 07:28 am (UTC)
I have a terrible feeling i should know what PCR is but i can't remember!
Margaretpling on July 25th, 2001 12:57 pm (UTC)
Polymerase Chain Reaction.

In case that still doesn't ring any bells - it's how you amplify DNA.
Andrewsagima on July 25th, 2001 01:07 pm (UTC)
I remembered about 3 seconds before the comment loaded on my hotmail :) Shouldn't forget things like that should i? :)